This work explores the e-beam treatment method for treating wastewater that contains reactive dyes, Reactive Red 198 (RR 198) and Reactive T. Blue 21 (RB 21) in combination with salts. The effects of e-beam treatment were compared and analyzed for changes in pH, chemical and biological oxygen demands (COD and BOD), colour and toxicity for particular reactive dye solutions and reactive dye effluent. Biodegradability enhancement with e-beam treatment was assessed in terms of the increased ratio of BOD/COD. It was found that the BOD/COD ratio of reactive mixed dye effluent rose by 13%, 54% and 61% respectively, when 5,10 and 20 kGy e-beam dose was applied. This rate is comparatively less than that of red and blue dye effluent. This difference observed is due to the presence of a stable and complex structure of chromophores in the effluent. The structural alterations in the irradiated samples were examined using UV-visible absorbance and FTIR spectra analysis, which confirmed the dye breakdown through chromophore sites. The fish toxicity studies verified that the byproducts generated after the irradiation were less toxic than non-irradiated dyehouse effluent. Furthermore, for simulated dye house effluent, the effectiveness of coagulation and flocculation with and without e-beam pretreatment was assessed using Jar test method. This recommends e-beam pre-treatment prior to coagulation-flocculation, which results in a 20% high rate of COD reduction. Overall study showed that electron beam method can be used as pretreatment option for coagulation-flocculation as well as for biodegradation in textile ETP plant to enhance the performance in terms of COD reduction efficiency, chemical saving in coagulation-flocculation and residence time reduction in biodegradation process.