This paper describes the use of the bioreduction process to synthesize silver nanoparticles (AgNPs). The aqueous extract of Tabernaemontana divaricta (pinwheel flower) and silver nitrate, which serve as a reducing agent and precursor salt, respectively, have been used in the synthesis of AgNPs. Using scanning electron microscopy and UV-visible spectroscopy, synthesized AgNPs have been characterized.The optical characteristics of AgNPs were assessed using UV-visible spectroscopy by means of the Surface Plasmon resonance (SPR) peak. This SPR peak appeared at 410 nm and suggested an average particle size of 40 to 60 nm. Using SEM examination, the AgNPs’ morphology was investigated. Using the Continuous Pad-Dry-Cure technique, these synthesized AgNPs were applied to cotton fabric. AgNP-deposited fabricswere then evaluated in terms of antibacterial activity and wash durability. The developed AgNP-deposited cotton fabric showed excellent antibacterial activity up to 10 numbers of soap washes.